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Protein Techniques is a one-semester course with four scheduled hours of laboratory exercises and one scheduled hour of lecture per week. This course is designed to train students to work independently and safely in the biotechnology laboratory by teaching comprehensive research techniques, methods for documentation and data analysis, and good laboratory practices. Students will learn and practice protein techniques including protein quantification using the Bradford method; size exclusion chromatography; ion exchange chromatography; affinity chromatography; SDS-PAGE and gel staining, drying, and annotation; western blotting and immunodetection; and the preparation and use of an ELISA. The students will be taught to use bioinformatics tools and resources that can assist them with their research and the interpretation of their results, including NCBI databases.
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