Chromatography

Chromatography is a powerful technique used by scientists to separate and analyze the components of a mixture. It is an essential tool for evaluating the safety, content, and quality of samples from food, environmental, pharmaceutical, biological and chemical sources. This course will discuss the theory of branches of chromatography - reverse and normal phase, ion, gas, size exclusion and gel permeation - and the components of the equipment used and how to prevent and troubleshoot instrumental issues. Recent development and trends will be discussed along with sample preparation techniques and method development. The laboratory component of the course allows students to gain practical hands-on training on HPLC, UPLC, LC-MS, IC and GC equipment to apply the theory, including calculations, and knowledge presented in lecture. Upon completion of this course, a student should be able to follow a Standard Operating Procedure, run samples on LC, IC and GC instrumentation, and collect, interpret, and report meaningful data.